Real-Time PCR Investigation of Potential Vectors, Reservoirs, and Shedding Patterns of Feline Hemotropic Mycoplasmas

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Real-time PCR investigation of potential vectors, reservoirs, and shedding patterns of feline hemotropic mycoplasmas.

Three hemotropic mycoplasmas have been identified in pet cats: Mycoplasma haemofelis, "Candidatus Mycoplasma haemominutum," and "Candidatus Mycoplasma turicensis." The way in which these agents are transmitted is largely unknown. Thus, this study aimed to investigate fleas, ticks, and rodents as well as saliva and feces from infected cats for the presence of hemotropic mycoplasmas, to gain insi...

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molecular characterization and phylogenetic analysis of feline hemotropic mycoplasmas

background: feline hemotropic mycoplasmas are parasites of erythrocytes and include three species, mycoplasma haemofelis, candidatus mycoplasma haemominutum and candidatus mycoplasma turicensis. diagnosis of the infection with these microorganisms can be carried out using conventional assays such as blood cytology. however, these assays have a low accuracy and a high rate of false-positive resu...

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lentiviral vectors titration using real-time pcr

lentiviral vectors (lvs) are useful vehicle for genetransfer to dividing and non-dividing cells and genetic manipulations. however, the use of lentiviruses in studies requires an accurate titration technique.quantitative real-time pcr (qpcr) is a sensitive technique for the indication and quantitation of retrovirals particles. in this study, we used the qpcr for lentiviral vector titeration. th...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2007

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.02977-06